Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA

(Protocol summary only for purposes of this preview site)

The double-stranded, closed-circular, replicative form (RF) of M13 DNA is present in high copy numbers in infected cells, and its physical characteristics are essentially identical to those of closed-circular plasmid DNAs. Any of the methods commonly used to purify plasmid DNAsilica gels (such as those sold by QIAGEN) and alkaline lysis (Ish-Horowicz and Burke 1981)can therefore be used to isolate M13 RF DNA.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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