Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Rapid Amplification of Sequences from the 3 Ends of mRNAs: 3-RACE

(Protocol summary only for purposes of this preview site)

cDNAs whose first-strand synthesis has been primed by oligo(dT) generally contain the 3 sequences of the RNA. However, cDNAs generated by internal gene-specific primers will usually lack one or both terminal regions of the parental mRNA. And alternate splicing or the presence of an internal A-rich tract can generate cDNA clones that lack 3 sequences. These sequences can be recovered using 3-RACE (Frohman 1993; Scotto-Lavino 2006b), which can also be used to map the 3 termini of families of mRNAs with alternative polyadenylation sites.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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