Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Phosphorylating the 5 Termini of Oligonucleotides Using T4 Polynucleotide Kinase

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Synthetic oligonucleotides lack phosphate groups at their 5 termini and are therefore easily radiolabeled by transferring the -32P from [-32P]ATP in a reaction catalyzed by bacteriophage T4 polynucleotide kinase. Oligonucleotides radiolabeled in this way may be used as hybridization probes and primers for DNA sequencing, 5-end mapping of mRNAs.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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