Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Transformation of the BAC Host with the RecA Vector

(Protocol summary only for purposes of this preview site)

This protocol outlines the steps for introducing the RecA plasmid into the BAC host cells, and their preparation for subsequent transformation with the reporter plasmid. BAC host cells are rendered chemically competent and transformed with the RecA plasmid, and transformants are selected for tetracycline resistance to ensure the presence of the RecA marker.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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