Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Separation of RNA according to Size: Electrophoresis of RNA through Agarose Gels Containing Formaldehyde

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Samples of RNA may be denatured by treatment with formamide and separated by electrophoresis through agarose gels containing formaldehyde. In this method, adapted from Lehrach et al. (1977), Goldberg (1980), Seed (1982), and Rosen et al. (1990), RNA is fractionated by electrophoresis through an agarose gel containing 2.2 M formaldehyde. For additional information, see the box Agarose Formaldehyde Gels.


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