Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Table of Contents

Expand All | Contract All

The Inoue Method for Preparation and Transformation of Competent E. coli: Ultracompetent Cells

(Protocol summary only for purposes of this preview site)

At its best, this method for preparing competent E. coli from Inoue et al. (1990) can challenge the efficiencies achieved by Hanahan (Hanahan 1983; Hanahan et al. 1991; and see Protocol 1). However, under standard laboratory conditions, efficiencies of 1 108 to 3 108 transformed colonies/g of plasmid DNA are more typical. The advantages of the procedure are that it is less finicky and more predictable than the original Hanahan (1983) method.


Previous   |   Next

Save 30% & Get Free Shipping!

Save 30% at checkout on our website.
(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Search for information about other protocols included in the book:

Read What Others Are Saying About Molecular Cloning:

Tom Maniatis wins Lasker

'Not Quite a Book Prize'

Get alerts about special offers from Cold Spring Harbor Laboratory Press free to your Inbox