Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Cloning in Plasmid Vectors: Directional Cloning

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Most plasmid vectors in common use contain multiple cloning sites that, in essence, are clusters of recognition sequences for many different restriction enzymes. Given the large variety of multiple cloning sites currently available (as many as 46 unique sites are present in some polylinkers, e.g., pSE280 from Life Technologies; and still longer polylinkers have been assembled; Brosius 1992), it is almost always possible to find a plasmid vector carrying unique restriction sites that are compatible with the termini of a particular fragment of foreign DNA.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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