Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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DNA Transfection by Electroporation

(Protocol summary only for purposes of this preview site)

Electroporation, which uses pulsed electrical fields, can be used to introduce DNA into a variety of animal cells (Neumann et al. 1982; Wong and Neumann 1982; Potter et al. 1984; Sugden et al. 1985; Toneguzzo et al. 1986; Tur-Kaspa et al. 1986), plant cells (Fromm et al. 1985, 1986; Ecker and Davis 1986), and bacteria. Electroporation works well with cell lines that are refractory to other transformation techniques, such as lipofection and calcium phosphateDNA coprecipitation. But, as with other transfection methods, the optimal conditions for electroporating DNA into untested cell lines must be determined empirically.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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