Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Concentrating and Desalting Nucleic Acids with Microconcentrators

(Protocol summary only for purposes of this preview site)

Ultrafiltration through microconcentrators is a rapid (15 min) procedure for (1) simultaneous desalting and concentrating small volumes of solutions containing DNA (or RNA); (2) recovering nucleic acids from agarose gels; or (3) purifying the products of PCRs. A major supplier of microconcentrators is Millipore Inc. (http://www.millipore.com/), and the protocols given below have been optimized for use with their Millicon cartridges. A range of microconcentrators fitted with anisotropic, hydrophilic-generated cellulose membranes of varying porosities is available.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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