Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Screening Colonies by PCR

(Protocol summary only for purposes of this preview site)

PCR, rather than the more labor-intensive minipreps, is often used to screen colonies of Escherichia coli for recombinant plasmids containing sequences of interest. When screening colonies for a DNA fragment amplified by PCR, the same primers used in the amplification reaction can be used for screening. To discover the orientation of the cloned fragment, a third primer can be used that binds to the plasmid sequences immediately adjacent to the insertion site and points toward the insert. This method can also be used to screen libraries for clones of interest (Israel 2006).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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