Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Solution-Phase Exome Capture

(Protocol summary only for purposes of this preview site)

This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. This protocol is the most commonly used approach for exome capture. Solid-phase methods were used at one time but have been superseded by solution-phase capture, in part because of the development of specialized reagents developed by various companies. Further issues and advantages of solution-phase exome capture are discussed in the chapter introduction under Targeted Sequencing: An Overview (see also Fig. 1 in the chapter introduction).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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