Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Cycle-Sequencing Reactions

(Protocol summary only for purposes of this preview site)

Cycle sequencing is used for direct sequencing of clones to identify a mutation or structure of interest. Here the template is typically prepared from plasmid minipreps (described in Protocol 1). For sequencing reactions, amplicons, prepared as described in Protocol 2, are sequenced individually with each of the two primers used in the LongAmp amplification reactions. Both template preparations use primers designed specifically for targetseither for direct sequencing or for validation.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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