Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Yeast Colony PCR

(Protocol summary only for purposes of this preview site)

Yeast colony PCR is used to amplify fragments from the yeast genome as well as transformed plasmids. Here it is used to verify that the correct DNA-bait is upstream of both reporters when preparing integrant strains in Protocol 1, to verify the identity of yeast transformed with DB-bait constructs in Protocol 2, and to amplify the inserts from interacting library clones during gap-repair retesting. This protocol takes 2 d to complete.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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