Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Sensitive Determination of Infectious Titer of rAAVs Using TCID50 End-Point Dilution and qPCR

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AAV recombinants are currently the vector of choice for many gene therapy applications. As experimental therapies progress to clinical trials, the need to characterize rAAVs accurately and reproducibly increases. Accurate determination of rAAV infectious titer is important for determining the activity of each vector lot and for ensuring lot-to-lot consistency. Early assays to determine rAAV infectious titer, such as the infectious center assay (Salvetti et al. 1998) and a hybridization-based 96-well TCID50 assay (Atkinson et al. 1998), suffer from high background levels, subjective calling of infectious events, and low sensitivity.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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