Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Expression Profiling by Microarray and RNA-seq

(Protocol summary only for purposes of this preview site)

Because there is no widely used software for analyzing RNA-seq data that has a graphical user interface, this protocol provides an example of analyzing microarray data using Babelomics (Medina et al. 2010; http://babelomics.bioinfo.cipf.es/). This analysis entails performing quantile normalization and then detecting differentially expressed genes associated with the transgenesis of a human oncogene c-Myc in mice. Finally, hierarchical clustering is performed on the differentially expressed genes using the Cluster program (Eisen et al. 1998; de Hoon et al. 2004; http://bonsai.hgc.jp/~mdehoon/software/cluster/), and the results are visualized using TreeView (Saldanha 2004; http://source forge.net/projects/jtreeview/files/).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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