Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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RNA-Seq: RNA Conversion to cDNA and Amplification

(Protocol summary only for purposes of this preview site)

The Ovation RNA-Seq Kit provides a fast and simple method for preparing amplified cDNA from total RNA. Amplification is initiated both at the 3 ends of the transcripts and across the entire range of transcribed sequences; thus, this approach is ideal for next-generation sequencing, because the reads are distributed across the transcript types. The amplified cDNA produced in this protocol is used to create libraries optimized for use in the Illumina Genome Analyzer II platform.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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