Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Precipitation of DNA with Ethanol

(Protocol summary only for purposes of this preview site)

DNA can be precipitated out of solution for the removal of salts and/or for resuspension in an alternative buffer. Either ethanol or isopropanol can be used to achieve this purpose; however, the use of ethanol is generally preferred (see the Discussion at the end of this protocol and the introduction to Protocol 5). Cations, provided as salts, are typically included to neutralize the negative charge of the DNA phosphate backbone. A listing of these with their respective stock and working concentrations is given in Table 1. The following method describes ethanol precipitation of DNA in microcentrifuge tubes.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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