Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Table of Contents

Expand All | Contract All

Expression of Cloned Genes Using the Baculovirus Expression System

(Protocol summary only for purposes of this preview site)

Almost all baculovirus expression systems use the procedures for insect cell transfection, baculovirus production, and protein expression given in the main portion of this protocol. Before transfection, standard molecular biology methods are used to place a target gene under control of a viral promoter carried in an E. coli vector. This construct is then transferred into the DNA of a baculovirus vector by homologous recombination. Although the major downstream steps remain the same, a variety of strategies are available for achieving recombination between the transfer vector and the viral DNA. As outlined in the introduction to this chapter, these approaches range from cotransfection to recombination in E. coli hosts or in vitro.


Previous   |   Next

Save 30% & Get Free Shipping!

Save 30% at checkout on our website.
(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Search for information about other protocols included in the book:

Read What Others Are Saying About Molecular Cloning:

Tom Maniatis wins Lasker

'Not Quite a Book Prize'

Get alerts about special offers from Cold Spring Harbor Laboratory Press free to your Inbox