Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Agarose Gel Electrophoresis

(Protocol summary only for purposes of this preview site)

Agarose is a linear polymer composed of alternating residues of D-galactose and L-galactose joined by -(13) and -(14) glycosidic linkages. Chains of agarose form helical fibers that aggregate into supercoiled structures with a radius of 2030 nm. Gelation of agarose results in a three-dimensional (3D) mesh of channels whose diameters range from 50 nm to >200 nm (Norton et al. 1986; for review, see Kirkpatrick 1990).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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