Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Labeling of DNA Probes by Polymerase Chain Reaction

(Protocol summary only for purposes of this preview site)

The polymerase chain reaction (see Chapter 7) can be used to produce both nonradiolabeled DNA probes and radiolabeled DNA probes with high specific activity. The advantages of the method include the following.

  • Defined segments of the target DNA can be amplified and labeled independently of the location or type of restriction sites.
  • There is no need to isolate fragments of DNA or to subclone them into vectors containing bacteriophage promoters.
  • Only small amounts of template DNA are required (210 ng or 1 fmol).
  • The specific activity of the radiolabeled amplified DNA can exceed 109 dpm/g.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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