Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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RNase Titration, Immunoprecipitation, and SDS-PAGE

(Protocol summary only for purposes of this preview site)

The CLIP protocol continues using cross-linked RNABP:RNA complexes from mixed lysates or cell pellets (Protocol 2). The material is treated with RNase to partially hydrolyze the bound RNA, and the complexes are immunoprecipitated using conditions optimized in Protocol 1. 3 Linkers are added, the RNA is radiolabeled, and the complexes are purified on SDS-PAGE.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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