Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Isolation of Total RNA from Zebrafish Embryos and Adults

(Protocol summary only for purposes of this preview site)

This protocol, which uses a monophasic lysis reagent, is a rapid method for isolating high-quality total RNA from multiple samples. The protocol is a general procedure that can be used for isolating RNA from zebrafish embryos at any developmental stage. Embryos can be lysed directly in the monophasic lysis reagent without prior dechorionation. Typical yields from 25 6-hpf (hours postfertilization) embryos are 58 g of RNA; 1015 g of RNA can be isolated from 25 24-hpf embryos.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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