Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Small-Scale Isolation of BAC DNA and Verification by PCR

(Protocol summary only for purposes of this preview site)

Small amounts of selected BAC DNAs are prepared from 5-mL cultures of the E. coli host transformed with the BAC clone. DNA is isolated by an adaptation of the alkaline lysis method described in Chapter 1, Protocol 1. (Alternatively, the QIAGEN spin miniprep kit may be used.) The yield is 0.10.4 g, and the BAC DNA is suitable for analysis by PCR. Considerations for clone selection are addressed in the Discussion section.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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