Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Table of Contents

Expand All | Contract All

Small-Scale Isolation of BAC DNA and Verification by PCR

(Protocol summary only for purposes of this preview site)

Small amounts of selected BAC DNAs are prepared from 5-mL cultures of the E. coli host transformed with the BAC clone. DNA is isolated by an adaptation of the alkaline lysis method described in Chapter 1, Protocol 1. (Alternatively, the QIAGEN spin miniprep kit may be used.) The yield is 0.10.4 g, and the BAC DNA is suitable for analysis by PCR. Considerations for clone selection are addressed in the Discussion section.


Previous   |   Next

Save 30% & Get Free Shipping!*

Save 30% at checkout on our website.
(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Search for information about other protocols included in the book:

Read What Others Are Saying About Molecular Cloning:

Tom Maniatis wins Lasker

'Not Quite a Book Prize'

Get alerts about special offers from Cold Spring Harbor Laboratory Press free to your Inbox

* Free shipping to individuals in U.S. and Canada only