Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Detection of DNA in Polyacrylamide Gels by Staining

(Protocol summary only for purposes of this preview site)

Unlike agarose gels, polyacrylamide gels cannot be cast in the presence of ethidium bromide because the dye inhibits polymerization of the acrylamide. However, ethidium bromide can be used to stain the polyacrylamide gel after electrophoresis. Because polyacrylamide quenches the fluorescence of the dye, the sensitivity with which DNA can be detected is somewhat diminished.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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