Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

(Protocol summary only for purposes of this preview site)

This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2-O-methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3-terminal cholesterol-conjugation facilitates delivery of ASOs into cells.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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