Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Table of Contents

Expand All | Contract All

Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

(Protocol summary only for purposes of this preview site)

This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2-O-methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3-terminal cholesterol-conjugation facilitates delivery of ASOs into cells.


Previous   |   Next

Save 30% & Get Free Shipping!*

Save 30% at checkout on our website.
(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Search for information about other protocols included in the book:

Read What Others Are Saying About Molecular Cloning:

Tom Maniatis wins Lasker

'Not Quite a Book Prize'

Get alerts about special offers from Cold Spring Harbor Laboratory Press free to your Inbox

* Free shipping to individuals in U.S. and Canada only