Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Library Quantification Using SYBR Green-qPCR

(Protocol summary only for purposes of this preview site)

To quantify complex DNA libraries, Kapa Biosystems has engineered a DNA polymerase specifically for SYBR Greenbased qPCR, enabling efficient amplification of targets such as GC-rich DNAs that present a challenge to wild-type DNA polymerases (see the introductory section in Chapter 1 for a discussion of SYBR Green). Kapa Library Quantification Kits contain this engineered polymerase to ensure robust amplification of longer fragments, across a broad range of GC content.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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