Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Cloning of the A Homology Arm into Reporter-Shuttle Vector

(Protocol summary only for purposes of this preview site)

The homology arm sequence, prepared as described in Protocol 5, is introduced by ligation into the shuttle vector carrying the reporter sequence to provide sites for recombination within the BAC clone (see Fig. 7 in the chapter introduction).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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