Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Attaching Phosphorylated Adaptors/Linkers to Blunt-Ended DNAs

(Protocol summary only for purposes of this preview site)

Adaptors and linkers are attached to the end of DNA fragments to facilitate their insertion into plasmid vectors for cloning and expression. To achieve maximal efficiency of ligation of adaptors, the termini of the target DNA are first polished with T4 DNA polymerase (see the information panels Adaptors and Linkers).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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