Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Creating Insertions or Deletions Using Overlap Extension PCR Mutagenesis

(Protocol summary only for purposes of this preview site)

Overlap extension PCR mutagenesis can be used for the generation of a specific point mutation, insertion, or deletion within a particular DNA sequence of interest. Overlap extension PCR mutagenesis requires relatively little preparation compared with other mutagenesis methods and does not require the use of restriction enzymes. Because of its versatility, the method has become widely used. Unlike methods of random mutagenesis, directed mutagenesis requires that the researcher already have a specific mutation in mind to implement.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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