Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Isolation of the RNA Tags, 5-Linker Ligation, and Reverse Transcription PCR Amplification

(Protocol summary only for purposes of this preview site)

This protocol describes purification of RNA tags by proteinase K digestion of the cross-linked protein, addition of a 5 linker to the RNA tags, and amplification of the product by RT-PCR. Use of this protocol adds another important purification step: sizing of the PCR products to enrich for those derived from RNA originally cross-linked to the desired RNABP.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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