Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Library Quantification Using PicoGreen Fluorometry

(Protocol summary only for purposes of this preview site)

This protocol describes the quantification of DNA using PicoGreen and a fluorometer to determine the concentration of DNA sample for downstream processing (see discussion of PicoGreen in the introductory section in Chapter 1). Because dye-based methods will not detect degraded or short DNA fragments, PicoGreen requires DNAs 50 bp, but can less reliably detect fragments as small as 20 bp.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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