Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Whole Genome: Automated, Nonindexed Library Preparation

(Protocol summary only for purposes of this preview site)

This protocol describes an automated procedure for constructing a nonindexed Illumina DNA library and relies on the use of a CyBio-SELMA automated pipetting machine, the Covaris E210 shearing instrument, and the epMotion 5075. With this method, genomic DNA fragments are produced by sonication, using high-frequency acoustic energy to shear DNA. Here, double-stranded DNA is fragmented when exposed to the energy of Adaptive Focused Acoustic Shearing (AFA; see the information panel Fragmenting of DNA). The resulting DNA fragments are ligated to adaptors, amplified by PCR, and subjected to size selection using magnetic beads. The product is suitable for use as template in whole-genome sequencing.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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