Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Detection of DNA in Polyacrylamide Gels by Autoradiography

(Protocol summary only for purposes of this preview site)

Bands of radioactive DNA separated by acrylamide gel electrophoresis may be detected by autoradiography. Analytical polyacrylamide gels containing radioactive DNA are usually fixed and dried before autoradiography. However, if bands of radioactive DNA are to be recovered from the gel, the gel should generally not be fixed or dried. In this case, omit Steps 13 and proceed directly to Step 4 to process the gel for autoradiography.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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