Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Table of Contents

Expand All | Contract All

High-Efficiency Yeast Transformation

(Protocol summary only for purposes of this preview site)

High-efficiency yeast transformation is used for integrations into YM4271 (Y1H DNA-bait generation), for transforming libraries of AD-prey clones into Y1H and Y2H bait strains, and for gap repair. Although this is a robust and dependable transformation protocol, occasionally transformation efficiency may be poor. Should that occur, refer to the Troubleshooting sections in this protocol and Protocol 3 for assistance. The protocol takes 2 d to complete.


Previous   |   Next

Save 30% & Get Free Shipping!*

Save 30% at checkout on our website.
(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Search for information about other protocols included in the book:

Read What Others Are Saying About Molecular Cloning:

Tom Maniatis wins Lasker

'Not Quite a Book Prize'

Get alerts about special offers from Cold Spring Harbor Laboratory Press free to your Inbox

* Free shipping to individuals in U.S. and Canada only