Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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High-Efficiency Yeast Transformation

(Protocol summary only for purposes of this preview site)

High-efficiency yeast transformation is used for integrations into YM4271 (Y1H DNA-bait generation), for transforming libraries of AD-prey clones into Y1H and Y2H bait strains, and for gap repair. Although this is a robust and dependable transformation protocol, occasionally transformation efficiency may be poor. Should that occur, refer to the Troubleshooting sections in this protocol and Protocol 3 for assistance. The protocol takes 2 d to complete.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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