Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Analysis of Cell Viability by the Lactate Dehydrogenase Assay

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A common method for determining cytotoxicity is based on measuring the activity of cytoplasmic enzymes released by damaged cells. Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme that is found in all cells. There are five different isoforms of lactate dehydrogenase present in different tissue types, and they differ in their quantity, specificity, and kinetics of enzyme action. But all of the different enzyme isoforms catalyze the common reaction of interconverting pyruvate to lactate accompanied by the oxidation of NADH to NAD (Fig. 1).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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