Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Preparation of dsRNAs by In Vitro Transcription

(Protocol summary only for purposes of this preview site)

This is a simple and widely used method for the preparation of dsRNA using a PCR templatebased, in vitro transcription reaction. The resulting dsRNA is then used to trigger RNAi in an appropriate cell or organism.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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