Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step RT-PCR

(Protocol summary only for purposes of this preview site)

RT-PCR is a powerful method to detect and synthesize cDNA copies of low-copy-number mRNAs. Two enzymes are used: reverse transcriptase to produce single-stranded cDNA copies, which are then used as templates in an amplification reaction catalyzed by a thermostable DNA polymerase. This protocol describes the traditional method of RT-PCR in which the two synthetic reactions are performed separately and sequentially. For this reason, the method is known as two-step RT-PCR.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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