Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Preparation of Small-Fragment Libraries for 454 Sequencing

(Protocol summary only for purposes of this preview site)

This protocol describes preparation of libraries of small DNA fragments for use in sequencing on the 454 Sequencing System (FLX or XLR). Fragmented genomic DNA is purified and ligated to adaptor sequences, then immobilized on specialized beads. The library consists of a set of single-stranded DNA fragments representing the complete span of the target DNA. After quality assessment and quantitation, the single-stranded library is ready for amplification by emPCR as described in Protocol 16.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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