Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Cloning PCR Products: TA Cloning

(Protocol summary only for purposes of this preview site)

The non-template-dependent terminal transferase activity inherent in nonproofreading DNA polymerases such as Taq provides a highly efficient method to clone PCR products. The enzyme adds a single, unpaired residuepreferentially an adenosyl residue (Clark 1988) to each 3 end of a double-stranded amplified product. The unpaired terminal (A) residues can pair with a linear T vector that carries an unpaired 3-thymidyl residue at each end (Holton and Graham 1991; Marchuk et al. 1991; for reviews, see Trower and Elgar 1994; Zhou and Gomez-Sanchez 2000).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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