Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Inhibiting miRNA Function by Antisense Oligonucleotides in Cultured Mammalian Cells

(Protocol summary only for purposes of this preview site)

Methods for transfecting antisense oligonucleotides (ASOs) into mammalian cells for suppression of miRNA function are described in this protocol. The effects of ASOs on the miRNA are evaluated by measuring the protein level of the putative miRNA target or the activity of a reporter bearing a 3 untranslated region (3 UTR) derived from the putative miRNA target. This protocol is designed for cells grown in 24-well plates. If other multiwell plates, flasks, or dishes of a different diameter are used, scale the cell density and reagent volumes according to the surface area of the well (see Table 1 of Protocol 2).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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