Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Purification of Labeled Oligonucleotides by Chromatography on a Sep-Pak C18 Column

(Protocol summary only for purposes of this preview site)

This protocol, which is a modification of procedures described by Lo et al. (1984), Sanchez-Pescador and Urdea (1984), and Zoller and Smith (1984), describes a method for the separation of radiolabeled oligonucleotides from unincorporated radiolabel that takes advantage of the reversible affinity of oligonucleotides for silica gel. This protocol can be used only to purify oligonucleotides carrying a 5-phosphate group, radiolabeled or unlabeled. It should be noted that ready-to-use Sep-Pak C18 chromatography cartridges are commercially available (e.g., Science Kit, Waters Division of Millipore).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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