Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Preparing Plasmid Subclones for Capillary Sequencing

(Protocol summary only for purposes of this preview site)

This protocol describes the preparation of plasmid DNA from bacterial cultures or from archived cultures, based on the standard miniprep method (see Chapter 1, Protocol 1). The resulting DNA is suitable in quantity and quality for use as template in capillary DNA sequencing as described in Protocol 3. An alternative method for preparing template is the amplification of a targeted region of DNA, described in Protocol 2. The protocol relies on the use of the Biomek automated liquid handling system.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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