Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Colony Lift Colorimetric Assay for -Galactosidase Activity

(Protocol summary only for purposes of this preview site)

The bacterial LacZ gene is a common reporter in yeast hybrid assays. This colony lift assay is used to detect -galactosidase expression in bait strains due to either autoactivity or expression induced by an AD-prey. This protocol takes 2 d to complete.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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