Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Table of Contents

Expand All | Contract All

Inverse PCR

(Protocol summary only for purposes of this preview site)

Standard PCR is used to amplify a segment of DNA that lies between two inward-pointing primers. In contrast, inverse PCR (also known as inverted or inside-out PCR) is used to amplify DNA sequences that flank one end of a known DNA sequence and for which no primers are available. The technique was developed independently by several groups (Ochman et al. 1988; Triglia et al. 1988; Silver and Keerikatte 1989), well before the advent of rapid and efficient DNA sequencing. These days, sequencing would in most cases be the method of choice to characterize an unknown segment of DNA. However, inverse PCR is still used extensively for rapid allelotyping and to determine the locations at which retroviruses, transgenes, and transposons are integrated into genomes.


Previous   |   Next

Save 30% & Get Free Shipping!

Save 30% at checkout on our website.
(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

Search for information about other protocols included in the book:

Read What Others Are Saying About Molecular Cloning:

Tom Maniatis wins Lasker

'Not Quite a Book Prize'

Get alerts about special offers from Cold Spring Harbor Laboratory Press free to your Inbox