Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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Whole Genome: Manual Library Preparation

(Protocol summary only for purposes of this preview site)

This protocol describes a manual approach for the preparation of genomic DNA libraries suitable for Illumina sequencing. Genomic DNA fragments produced by shearing by sonication are ligated to adaptors and amplified by PCR. The amplified DNA, separated by size and gel-purified, is suitable for use as template in whole-genome sequencing. Alternative methods using an automated approach are described in Protocols 5 and 6.


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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