Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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RNAi in Drosophila S2 Cells by siRNA Duplex Transfection

(Protocol summary only for purposes of this preview site)

This protocol describes an efficient method to trigger RNAi in Drosophila S2 cells by using transfection reagents to deliver siRNAs. This protocol is designed for cells grown in 24-well plates. If other multiwell plates, flasks, or dishes of a different diameter are used, scale the cell density and reagent volumes according to the surface area of the well (see Table 1).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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