Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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RNAi in Drosophila S2 Cells by dsRNA Transfection

(Protocol summary only for purposes of this preview site)

Although dsRNAs can be introduced into Drosophila S2 cells by simple soaking procedures, in our experience, transfection is more efficient. This dsRNA transfection protocol is designed for cells grown in 24-well plates. If other multiwell plates, flasks, or dishes of a different diameter are used, scale the cell density and reagent volumes according to the surface area of the well (see Table 1 of Protocol 3).


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(Limited time special offer.) Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook
Molecular Cloning Fourth Edition, A Laboratory Manual, by Michael R. Green and Joseph Sambrook

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